Megazyme Oligo-α-1,6-Glucosidase plus β-Galactosidase
High purity Oligo-α-1,6-Glucosidase (microbial) plus β-Galactosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Content: | 6,000 Units Oligo-α-1,6-Glucosidase plus 6,000 Units β-galactosidase |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 4oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 3 years at 4oC |
| Enzyme Activity | Oligo-1,6-α-Glucosidase, β-Galactosidase |
|---|---|
| EC Number | oligo-α-1,6-glucosidase: 3.2.1.10 β-galactosidase: 3.2.1.23 |
| CAZy Family | GH13, GH35 |
| CAS Number | oligo-α-1,6-glucosidase: 9032-15-9 β-galactosidase: 9013-11-2 |
| Synonyms | oligo-α-1,6-glucosidase: oligosaccharide 6-alpha-glucohydrolase β-galactosidase: beta-D-galactoside galactohydrolase |
| Source | oligo-α-1,6-glucosidase: Microbial β-galactosidase: Aspergillus niger |
| Molecular Weight | oligo-α-1,6-glucosidase: 67,300 β-galactosidase: 125,000 |
| Concentration | Oligo-α-1,6-glucosidase (2,000 U/mL) / β-galactosidase (2,000 U/mL) |
| Expression | Oligo-α-1,6-glucosidase: Recombinant from a Microbial source β-Glucosidase: Purified from Aspergillus niger |
| Specificity | This enzyme mixture gives complete hydrolysis of isomaltose and lactose under the defined assay conditions, with no hydrolysis of the trisaccharide, β-D-Fruf-(2,1)-β-D-Fruf-(2,1)-β-D-Fruf. |
| Specific Activity | Oligo-α-1,6-glucosidase (~ 170 U/mg) / β-galactosidase (~ 170 U/mg) |
| Unit Definition | Oligo-α-1,6-glucosidase: One Unit of oligo-α-1,6-glucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-D-glucopyranoside (5 mM) in sodium acetate buffer (50 mM), pH 4.5 at 40oC. β-galactosidase: One Unit of β-galactosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-galactoside (10 mM) in sodium acetate buffer (100 mM), pH 4.5 at 40oC. |
| Temperature Optima | 50oC |
| pH Optima | 4.5 |
| Application examples | Used for removal of isomaltose and lactose in the measurement of dietary fiber |
