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Megazyme’s chromogenic substrates provide the most convenient methods for the assay of hydrolytic enzymes but as the native substrates, polysaccharides and oligosaccharides can give the truest insight into their mechanism of action, active site requirement, binding affinities and activities. Polysaccharides can often be employed for reducing sugar or viscometric assays while oligosaccharides are widely used as chromatographic standards for HPLC/HPAEC-PAD analysis or as defined substrates in enzyme characterisation studies.
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