Product Code: Ceramic HyperD®

Descriptions:

• High capacity afffinity resin for IgG purification or depletion.
• High binding capacity of human and murine IgGs.
• High selectivity without non-specific binding.
• Column packing is faster than with conventional agarose beads.
• Unique, multi-point coupling chemistry provides a sorbent that exhibits very low leakage of recombinant Protein A.
• Excellent scalability.
• Prepared using a rigid proprietary ceramic bead. Recombinant Protein A is immobilized to a specially formulated hydrogel within the porous ceramic bead. The ceramic bead, the hydrogel, and the linkage used for the Prtotein A coupling are chemically stable over a broad range of conditions.

Applications:

• Isolation and purification of IgG from ascites fluid, cell culture supernatant, transgenic milk, and various animal sera
• Separation of IgG classes (Protein A does not interfere with human IgG3)
• Separation of Fc fragments from a mixture of Fc and Fab fragments obtained after enzymatic hydrolysis
• Purification of humanized or mouse monoclonal antibodies
• Serparation of immune complexes
• Purification of enzyme conjugates
• Removal of bovine IgG from hybridoma cell culture